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Our Technology

Our modular solution can be coupled to any commercial microscope to provide it with the imaging technology that we proposed.

We use dynamic full field optical coherence tomography (DFFOCT) to inspect any biological sample at high resolution, namely 400 nm in the transverse direction and 700 nm in the axial direction.

Our device generates an image in less than 5 seconds, allowing real time tracking of live samples. Coupled to a stage-top incubator, it enables timelapse and longitudinal imaging over an extended period of time, from hours to months.

The commercial prototype will be ready in summer 2025: it will have a reduced footprint and it will be sold together with a software that controls the instrument, the acquisition process and the data storage.

Compatible with 90% of commercial microscopes

THE PHYSICS BEHIND

OCT is the optical equivalent to ultrasound scanner. It measures light naturally backscattered by subcellular structures. Our interferometric detection enables measuring the axial position of cell organelles via an equivalent  of the photon time of flight with extreme precision (~10^-16s). But more importantly, by integrating all subcellular transport inside each cell, it tells us how cells transport their intracellular material, which is often cell specific and depends on how the cell generates and spends its energy (metabolism)/ or simply depends on cell metabolism.

HSV rendering in dynamic FFOCT

By looking at the displacements of cells organelles at short time scales, we can specifically detect structures that are actively transported.

Dynamic FFOCT allows quantitative imaging

Color scale reflects activity

Frequently asked questions

Why do your images have color if there is no fluorescence?

The colors are artificially assigned and are based on the fluctuation statistics of the intensity of the light backscattered by the sample. They are related to its metabolism

Do I need to prepare my sample to use your module?

Not at all! You can image your sample directly in your classic multiwell plate.

Does your solution come with a software?

Yes, the module is sold with a software that entirely handles the acquisition process, from data recording to image generation and storage.

Do you sell the module only?

Not only, we can provide additional pieces (i.e. optical table, microscope, incubator) upon request. A software solution to perform automatic analysis on the generated images will be launched soon.

Can we “rent” the module or do we have to buy it?

We could provide tailor made renting solution upon request.

They’ve already use it

“The technology proposed by Lutèce Dynamics allowed our team to live image zebrafish eye at subcellular resolution, without any fluorescent staining. All retinal structures can be easily identified.”

Filippo del BeneVision Institute (France)

“Lutece Dynamics' DFFOCT module enabled us to explore the cellular architecture of intestinal organoids with ease, without the use of labelling.”

Maxime MaheNantes University (France)

“I tested the experimental system on in vivo drosophila samples. I could get z-stacks of about 70 µm for a non-genetically modified sample. I could observe several different cell types, and multiple cellular events such as cell divisions. This paves the way to new interesting ideas to explore in vivo processes on model organisms in a label free manner. I thank Olivier Thouvenin and Salvatore Azzollini for their availability and their sharp knowledge of the technology.”

Léo ValonPasteur Institute (France)

“The ease of use of the Lutèce Dynamics's module makes it a great option for live imaging of dynamic cellular processes. I could image wild-type zebrafish embryos, with no fluorescence, at different speeds (up to 400Hz). The team was not only extremely knowledgeable, they went above and beyond to assist me with imaging my samples and answer all my questions. I could image independently at the end of our first session together. My experience with them and the module has been extremely positive.”

Lydia DjenouneMassachusetts General Hospital and Harvard Medical School (USA)

    Get in touch

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    Or if you prefer to write us directly, you can reach out at
    support@lutecedynamics.com